Magdalena Chrzanowska-Wodnicka, PhD Portrait

Magdalena Chrzanowska-Wodnicka, PhD, FAHA

 
Associate Investigator
Blood Research Institute
BloodCenter of Wisconsin
 
Assistant Adjunct Professor
Department of Pharmacology & Toxicology
Medical College of Wisconsin
 
Research Member
Medical College of Wisconsin Cancer Center
 
Education and training
MSc, Jagiellonian University, Krakow, Poland
PhD, University of North Carolina at Chapel Hill
 
Contact information
Phone: (414) 937-3890
Fax: (414) 937-6284

Thrombosis, Hemostasis and Vascular Biology
My laboratory studies the function of small G proteins in the cardiovascular system. We use transgenic mouse and zebrafish models for in vivo studies and a variety of biochemical, molecular and microscopy approaches to interrogate signaling by small GTPases in vascular cells ex vivo.
Small G protein Rap1 has been the main focus of research in my laboratory over the past few years. Rap1, evolutionarily conserved and ubiquitously expressed, is activated downstream from multiple cell surface receptors and regulates several basic cellular functions: adhesion, migration, polarity, differentiation and growth. Well studied in vitro, Rap1 functions in mammalian systems in vivo are still not very well understood.
 
Angiogenesis
Using Rap1b-knockout mice my laboratory has revealed a novel role of Rap1b in vivo: regulation of angiogenesis. In an in vivo neonatal retinal model and a Matrigel plug model we demonstrated that Rap1b-deficiency leads to a defect in angiogenesis, and through ex vivo and in vitro studies, we localized the defect to endothelial cells and have shown that endothelial proliferation and migration are affected by Rap1b-deficiency. At the molecular level, the underlying mechanism involves defective activation and signaling downstream from Vascular Endothelial Growth Factor (VEGF) Receptor 2. Current research in my laboratory is focused on elucidating molecular mechanisms through which Rap1 regulates endothelial cell responses to VEGF promoting angiogenesis and investigating the role of Rap1 in pathological angiogenesis in vivo.
 
Cardiac hypertrophy and hypertension
Studies from my laboratory indicate that Rap1b is required for maintenance of normal blood pressure as Rap1b-knockout mice are hypertensive and develop cardiac hypertrophy. This is a novel function of Rap1 and my laboratory is currently utilizing tissue-specific knockout mice to understand which physiological processes involved in regulation of blood pressure and normal cardiac function are regulated by Rap1.

Grants
NIH NHLBI: R01HL111582, “Rap1 in VEGF signaling in endothelial cells”


Sribalaji Lakshmikanthan, PhD

Postdoctoral Fellow

Selected Publications
  • Chrzanowska-Wodnicka, M., Smyth, S., Schoenwaelder, S.M. and White, G.C. "Rap1b is required for normal platelet function and hemostasis in mice." - Journal of Clinical Investigation 115:680-687, 2005.
  • Chrzanowska-Wodnicka, M., White, G.C., Kraus, A.E. and VanSluys, J. "Defective angiogenesis, endothelial migration and MAPK signaling in Rap1b-/- mice". - Blood 111:2647-56, 2008 (with "Inside Blood" commentary).
  • Chrzanowska-Wodnicka M. Regulation of angiogenesis by a small GTPase Rap1. Vascular Pharmacology 53:1-10, 2010.
  • Sobczak M, Dargatz J, Chrzanowska-Wodnicka M. Isolation and culture of murine microvascular endothelial cells. Journal of Visualized Experimentshttp://www.jove.com/index/details.stp?id=2316.
  • Lakshmikanthan S, Sobczak M, Chun CZ, Henschel A, Dargatz J, Ramchandran R, Chrzanowska-Wodnicka M. Rap1 regulates angiogenesis by a mechanism involving integrin αvβ3-dependent VEGFR2 activation. Blood 118:2015-2026, 2011 (with “Inside Blood“ commentary).

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