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Flow Cytometry Core
Research Core Labs & Facilities Director:
L. William Cashdollar, Ph.D.
Call: (414) 937-3847
To schedule instrument use, please contact Hope Campbell
Call: (414) 937-3843
E-mail: hope.campbell@bcw.edu
Overview and Core PersonnelThe Flow Cytometry Core contains 3 separate Becton Dickinson cytometer systems.
Research Core Labs & Facilities Director:
L. William Cashdollar, Ph.D.
Call: (414) 937-3847
Instrumentation and ApplicationsBD LSRII Flow Cytometer
- 3 lasers:
- -mW, air cooled 488nm Argon-Ion solid-state Laser
- 17-mW, air-cooled 633nm Helium-Neon (HeNe) gas laser
- 20-mW, air-cooled 355nm (UV) solid-state laser
- Optics and filters allow for signal detection in
- 4 colors off 488nm: D (530/30), C (575/26), B (695/40), A (780/60)
- 2 colors off 633nm: B (660/20), A (780/60)
- 2 colors off 355nm: B (440/40), A (530/30)
- Digital acquisition system, FACSDiva software
BD LSRII Special Order System
- 4 lasers
- 50mW 488nm laser
- 150mW 532nm laser
- 638 nm laser
- 50mW 405nm laser
- Optics and filters allow for signal detection in
- 2 colors off of 488nm, B (530/30), A (695/40)
- 5 colors off of 532nm, E (575/26), D (610/20), C (660/40), B (710/50), A (780/60)
- 3 colors off of 638nm, C (660/20), B (710/50), A (780/60)
- 2 colors off of 405nm, B (450/50), A (525/50)
- Digital acquisition system, FACSDiva software
BD FACSAria Flow Cytometer and Sorter
- Bench top, high speed cell sorter
- 3 lasers:
- 20-mW, air cooled 488nm Argon-Ion solid-state Laser
- 17-mW, air-cooled 633nm Helium-Neon (HeNe) gas laser
- 25-mW, air-cooled 407nm solid-state laser
- 4- way/plates/slides sorting option
- Digital acquisition system, FACSDiva software
Accuri C6
- 2 lasers
- 488nm
- 640nm
- Optics and filters allow for signal detection in
- FL1 533 / 30 nm (488nm)
- FL2 585 / 40 nm (488nm)
- FL3 >670 nm (488nm)
- FL4 675 / 25 nm (640nm)
- Digital acquisition system, CFlow Plus
Miltenyi Biotec Magnetic Cell Separation Workstation
This workstation consists of a stand and 4 separation magnets that can be reserved for use. The usually vast initial cell population can be much reduced and greatly enriched for the target cell, with little or no added abuse to the cells. This cuts sorting time and usually increases viability.
This workstation consists of a stand and 4 separation magnets that can be reserved for use. The usually vast initial cell population can be much reduced and greatly enriched for the target cell, with little or no added abuse to the cells. This cuts sorting time and usually increases viability.
SchedulingThe Flow Core at BloodCenter of Wisconsin's Blood Research Institute (BRI) provides these services to all its investigators and outsider users as capacity permits. Instruments are reserved on a first come, first served basis with priority given to BRI Investigators. FACScan users are encouraged to attend training with the core technologist. LSRII users are required to attend a free training session with the core technologist. All BD ARIA Sorts are operator assisted and therefore must be scheduled at least 48 hours in advance. As a courtesy, we require 24 hours notice to cancel a sort, so that time can be made available to other users.
To schedule instrument use, please contact Hope Campbell
Call: (414) 937-3843
E-mail: hope.campbell@bcw.edu
Sample PreparationPlease discuss your experimental protocol with us. Sample preparation is always critical to success, and some reagents are not recommended due to optical considerations. We need to determine if there are any special cytometer cleaning requirements. The handling of any infectious or otherwise dangerous materials should be discussed prior to booking time on any system.
